An ultrasensitive fluorescent probe for rapid determination of thiophenols
Lin, L (Lin, Lin)[ 2,3 ] ; Zeng, XQ (Zeng, Xiangqiong)[ 3 ] ; Shen, YN (Shen, Yuning)[ 2 ] ; Zhu, HL (Zhu, Hailiang)[ 2 ]*; Qian, Y (Qian, Yong)[ 1,2 ]*
[ 1 ] Nanjing Normal Univ, Coll Chem & Mat Sci, 1 Wunyuan Rd, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, 163 Xianlin Rd, Nanjing 210023, Jiangsu, Peoples R China
[ 3 ] Chinese Acad Sci, Shanghai Adv Res Inst, Green Chem Engn Technol Res Ctr, Shanghai 201210, Peoples R China
TALANTA, Apr.2017, 165, 321-325
An ultrasensitive fluorescent probe TPP was developed for the highly selective detection of thiophenols based on excited-state intramolecular proton transfer (ESIPT) mechanism, its synthesis through a simple straightforward combination of HBT fluorophore with 2,4-dinitrobenzene functional group. A kinetic study of TPP towards thiophenol displayed a fast response time (similar to 150 s) and significant turn-on fluorescence enhancement (similar to 60 fold). Selective and competitive experiments exhibited an excellent selectivity of TPP toward thiophenols over biothiols (Cys, GSH) and other aliphatic thiols or nucleophiles. Using this ultrasensitive probe (LOD, 1.05x 10(-8) M), we have successfully monitored and quantified highly toxic thiophenols in aqueous media and real-water samples.