On April 11, 2025, at 10:00 AM, Professor Zhu Xiaoli from Shanghai Tenth People's Hospital delivered an academic lecture entitled "New Technologies for Real-Scene Imaging of Cell Phenotypes" in Conference Room 226 of Huaxing Building, hosted by the School of Chemistry and Materials Science. Faculty members and students from relevant disciplines attended this academic event.

Zhu Xiaoli is the director of the Clinical Laboratory of Shanghai Tenth People's Hospital, the chief scientist of the National Key Research and Development Program, and a national-level young talent. He also holds academic part-time positions such as the deputy director of the Immunodeficiency Monitoring and Transformation Professional Committee of the China Human Health Science and Technology Promotion Association, the secretary-general of the Shanghai Clinical Laboratory Alliance, a member of the Molecular Diagnosis Subcommittee of the Shanghai Medical Association, and an editorial board member of Biomolecules. He is mainly engaged in the research of new medical testing technologies. He has successively presided over more than 20 scientific research projects at or above the provincial and ministerial level, including 5 National Natural Science Foundation projects. He has published more than 90 academic papers in authoritative journals such as Chem, Science Advances, Nature Communications, and JACS, among which more than 30 have an impact factor of over 10. His research on cell-diagnosis-related technologies has won honors such as the National Disruptive Technology Innovation Award and the Qipu Seed Award, and has been successfully transformed.

In this academic report, Professor Zhu Xiaoli systematically introduced the latest progress in the field of cell diagnosis. First, he explained in an easy-to-understand way the essential differences between new cell imaging technologies and traditional imaging technologies. Subsequently, he shared a series of innovative achievements obtained by his research group in the field of molecular encoding technology in recent years. Based on the powerful programmable properties of DNA molecules, the research team pioneered the development of computer-aided design hybridization chain reaction technology (CAD-HCR) internationally. This technology has successfully achieved high-precision imaging analysis of single-cell spatial proteomics. On this basis, the team further developed an amplifying fluorescence encoding technology with target-responsive characteristics (FLUCO), achieving the simultaneous in-situ detection of 51 biomarkers for the first time and setting a new record in this field. By integrating cutting-edge technologies such as fluorescence encoding, computer-aided design, and machine vision, the team also innovatively developed proximity-exchange-assisted chromaticity encoding technology (PREEM), which has been successfully applied to multiplexed detection of epigenetic transcriptomes, miRNA omics, and pathogenic microorganisms, providing a powerful technical support for tumor microenvironment research, single-cell heterogeneity analysis, and spatio-temporal medicine research. Finally, Professor Zhu looked ahead to the broad application prospects of these technologies in clinical diagnosis and basic research. During the interactive session, the participating teachers and students had a heated discussion on technical details and application prospects, and the academic atmosphere at the scene was strong.

This academic exchange activity built a high-level academic dialogue platform for the participating teachers and students. It not only provided valuable learning opportunities but also promoted interdisciplinary ideological collisions through in-depth academic discussions, increasing the opportunities for cross - disciplinary innovation and cooperation.