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Faculty

Guanbo Wang Associate Professor

Research Interests: 
  • Development of mass spectrometry-based method for characterizing protein higher-order structure and dynamics
  • Characterization of therapeutic proteins, including monoclonal antibodies (mAb) and antibody-drug conjugates (ADC)
  • Instrumentation
Bio: 

Educational Background

Ph.D.   Department of Chemistry, University of Massachusetts Amherst, USA (2013)

B.S.     College of Chemistry and Molecular Engineering, Peking University, China (2008)

 

Research Projects

We are currently conducting multiple projects under support of an NSFC grant and the NJNU start-up grant.

 

Highlighted Journal Articles

Protein complexes (Native Mass Spectrometry)

Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Frank J. Beurskens, Aran F. Labrijn, Deniz Ugurlar, Piet Gros, Janine Schuurman, Paul W. H. I. Parren*, Albert J. R. Heck*. Molecular basis of assembly and activation of complement component C1 in complex with Immunoglobulin G1 and antigen. Molecular Cell, 2016, 63(1), 135-145

Guanbo Wang, Alex J. Johnson, Igor A. Kaltashov*. Evaluation of Electrospray Ionization Mass Spectrometry as a Tool for Characterization of Small Soluble Protein Aggregates. Analytical Chemistry, 2012, 84(3), 1718-1724

 

Protein higher order structure and dynamics (Hydrogen Exchange Mass Spectrometry)

Guanbo Wang, Rinat R. Abzalimov, Cedric E. Bobst, Igor A. Kaltashov*. Conformer-specific characterization of non-native protein states using hydrogen exchange and top-down mass spectrometry. Proceedings of the National Academy of Sciences U.S.A., 2013, 110(50), 20087-20092

Guanbo Wang and Igor A. Kaltashov*. Approach to characterization of the higher order structure of disulfide-containing proteins using hydrogen/deuterium exchange and top-down mass spectrometry. Analytical Chemistry, 2014, 86(15), 7293-7298

 

Characterization of therapeutic proteins (Native Mass Spectrometry)

Yang Yang, Guanbo Wang (co-first author), Ting Song, Carlito Lebrilla, Albert J. R. Heck*. Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches. MABS, 2017, doi: 10.1080/19420862.2017.1290033

Andrey Dyachenko, Guanbo Wang, Mike E. Belov, Alexander Makarov, Rob N. de Jong, Ewald T. J. van den Bremer, Paul W. H. I. Parren, Albert J. R. Heck*. Tandem native mass-spectrometry on antibody-drug conjugates and submillion Da antibody-antigen protein assemblies on an Orbitrap EMR equipped with a high-mass quadrupole mass selector. Analytical Chemistry, 2015, 87(12), 6095-6102

 

Instrumentation

Guanbo Wang, Rinat R. Abzalimov, Igor A. Kaltashov*. Direct Monitoring of Heat-Stressed Biopolymers with Temperature-Controlled Electrospray Ionization Mass Spectrometry. Analytical Chemistry, 2011, 83(8), 2870-2876 (accelerated article)

 

    (i) Book Translation

Guanbo Wang and Yuguo Ma. Chinese Edition of Chemical Demonstrations, by Basam Z. Shakhashiri. Peking University Press: Beijing, China, 2013 (ISBN: 9787301221464/O.0915)

    (ii) Book Chapters

Igor A. Kaltashov, Rinat R. Abzalimov, Guanbo Wang and Cedric E. Bobst, Top-down hydrogen/deuterium exchange mass spectrometry. In: Hydrogen-Deuterium Exchange Mass Spectrometry: Fundamentals, Techniques and Applications, edited by D. D. Weis. John Wiley & Sons, Inc. 2015, (ISBN: 978-1-118-61649-9)

Guanbo Wang. Formaldehyde the Swordsman. Book chapter in: The Republic of Molecules, edited by Y. Ma and F. Yu. Knowledge Publishing House: Beijing, China, 2009, pp. 102-108 (ISBN: 978-7-501-55629-8)

 

  •  

U.S. Patent Granted:   Temperature-controlled electrospray ionization source and methods of use thereof, 2014, Patent Number: US8766179

 

Honors and Awards

2014    Nomination for CGS/ProQuest Distinguished Dissertation Award, Council of Graduate Schools, USA

2013    Attendance as a Young Researcher at the 63rd Lindau Nobel Laureate Meeting, and the travel grant, Lindau, Germany

2012    Graduate School Fellowship, UMass-Amherst, USA

2011    Marvin D. Rausch Scholarship Award, Chemistry Dept., UMass-Amherst, USA

2008    Valedictorian, Commencement of College of Chemistry, Peking University, China

2008    Diploma with Honors, Peking University, China

     2016    Editorial Board Member, Analytical Instrumentation (ISSN: 1001-232X)

 

 

Contact

Tel.: +86-025-85891319

Email: guanbowang@njnu.edu.cn