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Faculty

Guanbo Wang Professor

Academic Area: 
Bioanalytical Chemistry
Research Interests: 
  • Development of mass spectrometry-based method for characterizing protein higher-order structure in relation to the biological function
  • Biophysical characterization of therapeutic proteins, including monoclonal antibodies (mAb)
  • Instrumentation
Bio: 

Professional Background

2017-present Professor, School of Chemistry and Materials Science, Nanjing Normal University

2016-2017     Associate Professor, School of Chemistry and Materials Science, Nanjing Normal University

2013-2016     Researcher, Department of Chemistry, Utrecht University, and Netherlands Proteomics Centre

2008-2013     Ph.D., Department of Chemistry, University of Massachusetts Amherst, USA

2004-2008     B.S., College of Chemistry and Molecular Engineering, Peking University, China

We use protein mass spectrometry (MS) as the primary tool to develop biophysical/analytical techniques and instruments for analysis of natural proteins, protein complexes and protein therapeutics, with emphasis on (1) biological functions of macromolecular assemblies; (2) protein conformation and dynamics; (3) post-translational modification and stability of protein therapeutics. We develope innovative methods based-on strategies including native MS and hydrogen exchange MS to characterize protein structure, interaction and dynamics in detail, and applied these techniques to study mechanisms of biochemical reactions, protein denaturation/aggregation, and efficacy and stability of protein therapeutics, etc. 

Research Projects

We are currently conducting multiple projects under support of grants from NSFC, China Association for Science and Technology, and NJNU.

Highlighted Journal Articles

Yue Shen, Xiuxiu Zhao, Guanbo Wang*, David D. Y. Chen*, Differential Hydrogen/Deuterium Exchange during Proteoform Separation Enables Characterization of Conformational Differences between Coexisting Protein States, Analytical Chemistry, 2019, 91(6): 3805-3809

Wenjun Tong, Guanbo Wang*, How can native mass spectrometry contribute to characterization of biomacromolecular higher-order structure and interactions? Methods, 2018, 144: 3-13

Xiuxiu Zhao#, Yue Shen#, Wenjun Tong, Guanbo Wang*, David D. Y. Chen*, Deducing Disulfide Patterns of Cysteine-rich Proteins Using Signature Fragments Produced by Top-down Mass Spectrometry, Analyst, 2018, 143(4): 817-823

Guanbo Wang*, Pavel V. Bondarenko, Igor A. Kaltashov, Multi-step Conformational Transitions in Heat-treated Protein Therapeutics can be Monitored in Real Time with Temperature-controlled Electrospray Ionization Mass Spectrometry, Analyst, 2018, 143(3): 670-677

Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Paul W. H. I. Parren, Albert J. R. Heck*. Enhancing accuracy in molecular weight determination of highly heterogeneously glycosylated proteins by native tandem mass spectrometry. Analytical Chemistry, 2017, 89(9): 4793-4797

Yang Yang#, Guanbo Wang#, Ting Song, Carlito Lebrilla, Albert J. R. Heck*. Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches. mAbs, 2017, 9(4): 638-645

Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Frank J. Beurskens, Aran F. Labrijn, Deniz Ugurlar, Piet Gros, Janine Schuurman, Paul W. H. I. Parren*, Albert J. R. Heck*. Molecular basis of assembly and activation of complement component C1 in complex with Immunoglobulin G1 and antigen. Molecular Cell, 2016, 63(1): 135-145

Guanbo Wang and Igor A. Kaltashov*. Approach to characterization of the higher order structure of disulfide-containing proteins using hydrogen/deuterium exchange and top-down mass spectrometry. Analytical Chemistry, 2014, 86(15): 7293-7298

Guanbo Wang, Rinat R. Abzalimov, Cedric E. Bobst, Igor A. Kaltashov*. Conformer-specific characterization of non-native protein states using hydrogen exchange and top-down mass spectrometry. Proceedings of the National Academy of Sciences U.S.A., 2013, 110(50): 20087-20092

Guanbo Wang, Alex J. Johnson, Igor A. Kaltashov*. Evaluation of Electrospray Ionization Mass Spectrometry as a Tool for Characterization of Small Soluble Protein Aggregates. Analytical Chemistry, 2012, 84(3): 1718-1724

Guanbo Wang, Rinat R. Abzalimov, Igor A. Kaltashov*. Direct Monitoring of Heat-Stressed Biopolymers with Temperature-Controlled Electrospray Ionization Mass Spectrometry. Analytical Chemistry, 2011, 83(8): 2870-2876 (accelerated article)

    Books

    (i) Book Translation

Guanbo Wang and Yuguo Ma. Chinese Edition of Chemical Demonstrations, by Basam Z. Shakhashiri. Peking University Press: Beijing, China, 2013 (ISBN: 9787301221464)

    (ii) Book Chapters

Igor A. Kaltashov, Cedric E. Bobst, Jake Pawlowski, and Guanbo Wang, Characterization of the higher order structure in the discovery and development of protein therapeutics. In: Mass Spectrometry in Biopharmaceutical and Emerging Drug Modalities, edited by Mark Bolgar. Royal Society of Chemistry. 2019 (ISBN: 978-1-782-62975-7)

Igor A. Kaltashov, Rinat R. Abzalimov, Guanbo Wang and Cedric E. Bobst, Top-down hydrogen exchange mass spectrometry. In: Hydrogen Exchange Mass Spectrometry: Fundamentals, Techniques and Applications, edited by D. D. Weis. John Wiley & Sons, Inc. 2015, (ISBN: 978-1-118-61649-9)

Guanbo Wang. Formaldehyde the Swordsman. In: The Republic of Molecules, edited by Y. Ma and F. Yu. Knowledge Publishing House: Beijing, China, 2009, pp. 102-108 (ISBN: 978-7-501-55629-8)

    US Patent

U.S. Patent Granted: Temperature-controlled electrospray ionization source and methods of use thereof, 2014, Patent Number: US8766179

 

Honors and Awards

2017    Young Elite Scientist Sponsorship Program, China Association for Science and Technology

2017    100-talent Program, Nanjing Normal University

2014    Nomination for CGS/ProQuest Distinguished Dissertation Award, Council of Graduate Schools, USA

2013    Attendance as a Young Researcher at the 63rd Lindau Nobel Laureate Meeting, and the travel grant, Lindau, Germany

2012    Graduate School Fellowship, UMass-Amherst, USA

2011    Marvin D. Rausch Scholarship, Chemistry Dept., UMass-Amherst, USA

2008    Valedictorian, Commencement of College of Chemistry, Peking University, China

2008    Diploma with Honors, Peking University, China

     

Professional Service

2017    Editorial advisory group member, Cambridge Scholars Publishing, UK

2016    Editorial board member, Analytical Instrumentation (ISSN: 1001-232X)

 

Contact

Tel.: +86-025-85891319

Email: guanbowang[at]njnu[dot]edu[dot]cn

Skype: chemrookie