Skip to content Skip to navigation

Faculty

Guanbo Wang Professor

Academic Area: 
Bioanalytical Chemistry
Research Interests: 
  • Development of mass spectrometry-based method for characterizing protein higher-order structure in relation to the biological function
  • Characterization of therapeutic proteins, including monoclonal antibodies (mAb) and antibody-drug conjugates (ADC)
  • Instrumentation
Bio: 

Professional Background

2017-present Professor, School of Chemistry and Materials Science, Nanjing Normal University

2016-2017     Associate Professor, School of Chemistry and Materials Science, Nanjing Normal University

2013-2016     Researcher, Department of Chemistry, Utrecht University, and Netherlands Proteomics Centre

2008-2013     Ph.D., Department of Chemistry, University of Massachusetts Amherst, USA

2004-2008     B.S., College of Chemistry and Molecular Engineering, Peking University, China

Wang uses protein mass spectrometry (MS) as the primary tool to develop analytical techniques and instruments for analysis of protein complexes, protein higher-order structure and protein therapeutics, with emphasis on (1) biological functions of macromolecular assemblies; (2) protein denaturation and aggregation; (3) post-translational modification and stability of protein therapeutics and their impact on the efficacy and safety. He developed innovative methods based-on strategies including native MS and hydrogen exchange MS to characterize protein structure, interaction and dynamics in detail, and applied these methodologies in fields ranging from immunology to pharmaceutical research. Wang has published first-authored research articles in journals including PNAS, Mol. Cell and Anal. Chem., one book translation and multiple book chapters. Wang was granted one U.S. patent on his intention of MS ion source used for accessing protein stability. Wang is currently serving in the Editorial Advisory Board of Cambridge Scholars Publishing and Editorial Board of a peer-reviewed journal Analytical Instrumentation.

Research Projects

We are currently conducting multiple projects under support of an NSFC grant and the NJNU start-up grant.

Highlighted Journal Articles

Protein higher order structure and dynamics

Guanbo Wang, Rinat R. Abzalimov, Cedric E. Bobst, Igor A. Kaltashov*. Conformer-specific characterization of non-native protein states using hydrogen exchange and top-down mass spectrometryProceedings of the National Academy of Sciences U.S.A., 2013, 110(50), 20087-20092

Guanbo Wang and Igor A. Kaltashov*Approach to characterization of the higher order structure of disulfide-containing proteins using hydrogen/deuterium exchange and top-down mass spectrometryAnalytical Chemistry, 2014, 86(15), 7293-7298

Protein interaction (Native Mass Spectrometry)

Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Frank J. Beurskens, Aran F. Labrijn, Deniz Ugurlar, Piet Gros, Janine Schuurman, Paul W. H. I. Parren*, Albert J. R. Heck*. Molecular basis of assembly and activation of complement component C1 in complex with Immunoglobulin G1 and antigen. Molecular Cell, 2016, 63(1), 135-145

Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Paul W. H. I. Parren, Albert J. R. Heck*. Enhancing accuracy in molecular weight determination of highly heterogeneously glycosylated proteins by native tandem mass spectrometry. Analytical Chemistry, 2017, 89(9), 4793-4797

Guanbo Wang, Alex J. Johnson, Igor A. Kaltashov*. Evaluation of Electrospray Ionization Mass Spectrometry as a Tool for Characterization of Small Soluble Protein Aggregates. Analytical Chemistry, 2012, 84(3), 1718-1724

Characterization of therapeutic proteins

Yang Yang#, Guanbo Wang#, Ting Song, Carlito Lebrilla, Albert J. R. Heck*. Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches. mAbs, 2017, 9(4), 638-645.

Andrey Dyachenko, Guanbo Wang, Mike E. Belov, Alexander Makarov, Rob N. de Jong, Ewald T. J. van den Bremer, Paul W. H. I. Parren, Albert J. R. Heck*. Tandem native mass-spectrometry on antibody-drug conjugates and submillion Da antibody-antigen protein assemblies on an Orbitrap EMR equipped with a high-mass quadrupole mass selector. Analytical Chemistry, 2015, 87(12), 6095-6102

Instrumentation

Guanbo Wang, Rinat R. Abzalimov, Igor A. Kaltashov*. Direct Monitoring of Heat-Stressed Biopolymers with Temperature-Controlled Electrospray Ionization Mass Spectrometry. Analytical Chemistry, 2011, 83(8), 2870-2876 (accelerated article)

    Books

    (i) Book Translation

Guanbo Wang and Yuguo Ma. Chinese Edition of Chemical Demonstrations, by Basam Z. Shakhashiri. Peking University Press: Beijing, China, 2013 (ISBN: 9787301221464/O.0915)

    (ii) Book Chapters

Igor A. Kaltashov, Rinat R. Abzalimov, Guanbo Wang and Cedric E. Bobst, Top-down hydrogen/deuterium exchange mass spectrometry. In: Hydrogen-Deuterium Exchange Mass Spectrometry: Fundamentals, Techniques and Applications, edited by D. D. Weis. John Wiley & Sons, Inc. 2015, (ISBN: 978-1-118-61649-9)

Guanbo Wang. Formaldehyde the Swordsman. Book chapter in: The Republic of Molecules, edited by Y. Ma and F. Yu. Knowledge Publishing House: Beijing, China, 2009, pp. 102-108 (ISBN: 978-7-501-55629-8)

    US Patent

U.S. Patent Granted:   Temperature-controlled electrospray ionization source and methods of use thereof, 2014, Patent Number: US8766179

Honors and Awards

2014    Nomination for CGS/ProQuest Distinguished Dissertation Award, Council of Graduate Schools, USA

2013    Attendance as a Young Researcher at the 63rd Lindau Nobel Laureate Meeting, and the travel grant, Lindau, Germany

2012    Graduate School Fellowship, UMass-Amherst, USA

2011    Marvin D. Rausch Scholarship Award, Chemistry Dept., UMass-Amherst, USA

2008    Valedictorian, Commencement of College of Chemistry, Peking University, China

2008    Diploma with Honors, Peking University, China

     2016    Editorial Board Member, Analytical Instrumentation (ISSN: 1001-232X)

 

Contact

Tel.: +86-025-85891319

Email: guanbowang@njnu.edu.cn

Skype: chemrookie