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Faculty

Guanbo Wang Professor

Academic Area: 
Bioanalytical Chemistry
Research Interests: 
  • Development of mass spectrometry-based method for characterizing protein higher-order structure in relation to the biological function
  • Characterization of therapeutic proteins, including monoclonal antibodies (mAb) and antibody-drug conjugates (ADC)
  • Instrumentation
Bio: 

Professional Background

2017-present Professor, School of Chemistry and Materials Science, Nanjing Normal University

2016-2017     Associate Professor, School of Chemistry and Materials Science, Nanjing Normal University

2013-2016     Researcher, Department of Chemistry, Utrecht University, and Netherlands Proteomics Centre

2008-2013     Ph.D., Department of Chemistry, University of Massachusetts Amherst, USA

2004-2008     B.S., College of Chemistry and Molecular Engineering, Peking University, China

Wang uses protein mass spectrometry (MS) as the primary tool to develop analytical techniques and instruments for analysis of protein complexes, protein higher-order structure and protein therapeutics, with emphasis on (1) biological functions of macromolecular assemblies; (2) protein denaturation and aggregation; (3) post-translational modification and stability of protein therapeutics, and their impact on efficacy and safety. He developed innovative methods based-on strategies including native MS and hydrogen exchange MS to characterize protein structure, interaction and dynamics in detail, and applied these techniques to study mechanisms of immunoreactions and protein denaturation/aggregation, efficacy and stability of protein therapeutics, etc.. Wang has published articles with first- or corresponding authorship in journals including PNAS, Mol. Cell, Anal. Chem. and mAbs, one book translation and multiple book chapters. He was granted one U.S. patent on his intention of MS ion source used for accessing protein stability. Wang is currently serving in the Editorial Advisory Board of Cambridge Scholars Publishing and Editorial Board of a peer-reviewed journal Analytical Instrumentation.

Research Projects

We are currently conducting multiple projects under support of an NSFC grant and the NJNU start-up grant.

Highlighted Journal Articles

Protein higher order structure and dynamics

Guanbo Wang, Rinat R. Abzalimov, Cedric E. Bobst, Igor A. Kaltashov*. Conformer-specific characterization of non-native protein states using hydrogen exchange and top-down mass spectrometry. Proc. Natl. Acad. Sci. U.S.A., 2013, 110(50), 20087-20092

Guanbo Wang and Igor A. Kaltashov*. Approach to characterization of the higher order structure of disulfide-containing proteins using hydrogen/deuterium exchange and top-down mass spectrometry. Anal. Chem., 2014, 86(15), 7293-7298

Xiuxiu Zhao, Yue Shen, Wenjun Tong, Guanbo Wang*, David D. Y. Chen*. Deducing disulfide patterns of cysteine-rich proteins using signature fragments produced by top-down mass spectrometry. Analyst, 2018, 143(4), 817-823.

Protein interaction (Native Mass Spectrometry)

Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Frank J. Beurskens, Aran F. Labrijn, Deniz Ugurlar, Piet Gros, Janine Schuurman, Paul W. H. I. Parren*, Albert J. R. Heck*. Molecular basis of assembly and activation of complement component C1 in complex with Immunoglobulin G1 and antigen. Mol. Cell, 2016, 63(1), 135-145

Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Paul W. H. I. Parren, Albert J. R. Heck*. Enhancing accuracy in molecular weight determination of highly heterogeneously glycosylated proteins by native tandem mass spectrometry. Anal. Chem., 2017, 89(9), 4793-4797

Guanbo Wang, Alex J. Johnson, Igor A. Kaltashov*. Evaluation of Electrospray Ionization Mass Spectrometry as a Tool for Characterization of Small Soluble Protein Aggregates. Anal. Chem., 2012, 84(3), 1718-1724

Characterization of therapeutic proteins

Guanbo Wang*, Pavel V. Bondarenko, Igor A. Kaltashov. Multi-step conformational transitions in heat-treated protein therapeutics can be monitored in real time with temperature-controlled electrospray ionization mass spectrometry. Analyst, 2018, 143(3), 670-677.

Yang Yang#, Guanbo Wang#, Ting Song, Carlito Lebrilla, Albert J. R. Heck*. Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches. mAbs, 2017, 9(4), 638-645.

Instrumentation

Guanbo Wang, Rinat R. Abzalimov, Igor A. Kaltashov*. Direct Monitoring of Heat-Stressed Biopolymers with Temperature-Controlled Electrospray Ionization Mass Spectrometry. Anal. Chem., 2011, 83(8), 2870-2876 (accelerated article)

    Books

    (i) Book Translation

Guanbo Wang and Yuguo Ma. Chinese Edition of Chemical Demonstrations, by Basam Z. Shakhashiri. Peking University Press: Beijing, China, 2013 (ISBN: 9787301221464)

    (ii) Book Chapters

Igor A. Kaltashov, Rinat R. Abzalimov, Guanbo Wang and Cedric E. Bobst, Top-down hydrogen exchange mass spectrometry. In: Hydrogen Exchange Mass Spectrometry: Fundamentals, Techniques and Applications, edited by D. D. Weis. John Wiley & Sons, Inc. 2015, (ISBN: 978-1-118-61649-9)

Guanbo Wang. Formaldehyde the Swordsman. In: The Republic of Molecules, edited by Y. Ma and F. Yu. Knowledge Publishing House: Beijing, China, 2009, pp. 102-108 (ISBN: 978-7-501-55629-8)

    US Patent

U.S. Patent Granted: Temperature-controlled electrospray ionization source and methods of use thereof, 2014, Patent Number: US8766179

 

Honors and Awards

2017    Young talents supporting program, China Association for Science and Technology

2017    100-talent Program, Nanjing Normal University

2014    Nomination for CGS/ProQuest Distinguished Dissertation Award, Council of Graduate Schools, USA

2013    Attendance as a Young Researcher at the 63rd Lindau Nobel Laureate Meeting, and the travel grant, Lindau, Germany

2012    Graduate School Fellowship, UMass-Amherst, USA

2011    Marvin D. Rausch Scholarship, Chemistry Dept., UMass-Amherst, USA

2008    Valedictorian, Commencement of College of Chemistry, Peking University, China

2008    Diploma with Honors, Peking University, China

     

Professional Service

2017    Editorial advisory group member, Cambridge Scholars Publishing, UK

2016    Editorial board member, Analytical Instrumentation (ISSN: 1001-232X)

 

Contact

Tel.: +86-025-85891319

Email: guanbowang@njnu.edu.cn

Skype: chemrookie