- Development of mass spectrometry-based method for characterizing protein higher-order structure in relation to the biological function
- Characterization of therapeutic proteins, including monoclonal antibodies (mAb) and antibody-drug conjugates (ADC)
2017-present Professor, School of Chemistry and Materials Science, Nanjing Normal University
2016-2017 Associate Professor, School of Chemistry and Materials Science, Nanjing Normal University
2013-2016 Researcher, Department of Chemistry, Utrecht University, and Netherlands Proteomics Centre
2008-2013 Ph.D., Department of Chemistry, University of Massachusetts Amherst, USA
2004-2008 B.S., College of Chemistry and Molecular Engineering, Peking University, China
Wang uses protein mass spectrometry (MS) as the primary tool to develop analytical techniques and instruments for analysis of protein complexes, protein higher-order structure and protein therapeutics, with emphasis on (1) biological functions of macromolecular assemblies; (2) protein denaturation and aggregation; (3) post-translational modification and stability of protein therapeutics, and their impact on efficacy and safety. He developed innovative methods based-on strategies including native MS and hydrogen exchange MS to characterize protein structure, interaction and dynamics in detail, and applied these techniques to study mechanisms of immunoreactions and protein denaturation/aggregation, efficacy and stability of protein therapeutics, etc.. Wang has published articles with first- or corresponding authorship in journals including PNAS, Mol. Cell, Anal. Chem. and mAbs, one book translation and multiple book chapters. He was granted one U.S. patent on his intention of MS ion source used for accessing protein stability. Wang is currently serving in the Editorial Advisory Board of Cambridge Scholars Publishing and Editorial Board of a peer-reviewed journal Analytical Instrumentation.
We are currently conducting multiple projects under support of an NSFC grant and the NJNU start-up grant.
Protein higher order structure and dynamics
Guanbo Wang, Rinat R. Abzalimov, Cedric E. Bobst, Igor A. Kaltashov*. Conformer-specific characterization of non-native protein states using hydrogen exchange and top-down mass spectrometry. Proc. Natl. Acad. Sci. U.S.A., 2013, 110(50), 20087-20092
Guanbo Wang and Igor A. Kaltashov*. Approach to characterization of the higher order structure of disulfide-containing proteins using hydrogen/deuterium exchange and top-down mass spectrometry. Anal. Chem., 2014, 86(15), 7293-7298
Xiuxiu Zhao, Yue Shen, Wenjun Tong, Guanbo Wang*, David D. Y. Chen*. Deducing disulfide patterns of cysteine-rich proteins using signature fragments produced by top-down mass spectrometry. Analyst, 2018, 143(4), 817-823.
Protein interaction (Native Mass Spectrometry)
Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Frank J. Beurskens, Aran F. Labrijn, Deniz Ugurlar, Piet Gros, Janine Schuurman, Paul W. H. I. Parren*, Albert J. R. Heck*. Molecular basis of assembly and activation of complement component C1 in complex with Immunoglobulin G1 and antigen. Mol. Cell, 2016, 63(1), 135-145
Guanbo Wang, Rob N. de Jong, Ewald T. J. van den Bremer, Paul W. H. I. Parren, Albert J. R. Heck*. Enhancing accuracy in molecular weight determination of highly heterogeneously glycosylated proteins by native tandem mass spectrometry. Anal. Chem., 2017, 89(9), 4793-4797
Guanbo Wang, Alex J. Johnson, Igor A. Kaltashov*. Evaluation of Electrospray Ionization Mass Spectrometry as a Tool for Characterization of Small Soluble Protein Aggregates. Anal. Chem., 2012, 84(3), 1718-1724
Characterization of therapeutic proteins
Guanbo Wang*, Pavel V. Bondarenko, Igor A. Kaltashov. Multi-step conformational transitions in heat-treated protein therapeutics can be monitored in real time with temperature-controlled electrospray ionization mass spectrometry. Analyst, 2018, 143(3), 670-677.
Yang Yang#, Guanbo Wang#, Ting Song, Carlito Lebrilla, Albert J. R. Heck*. Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches. mAbs, 2017, 9(4), 638-645.
Guanbo Wang, Rinat R. Abzalimov, Igor A. Kaltashov*. Direct Monitoring of Heat-Stressed Biopolymers with Temperature-Controlled Electrospray Ionization Mass Spectrometry. Anal. Chem., 2011, 83(8), 2870-2876 (accelerated article)
(i) Book Translation
Guanbo Wang and Yuguo Ma. Chinese Edition of Chemical Demonstrations, by Basam Z. Shakhashiri. Peking University Press: Beijing, China, 2013 (ISBN: 9787301221464)
(ii) Book Chapters
Igor A. Kaltashov, Rinat R. Abzalimov, Guanbo Wang and Cedric E. Bobst, Top-down hydrogen exchange mass spectrometry. In: Hydrogen Exchange Mass Spectrometry: Fundamentals, Techniques and Applications, edited by D. D. Weis. John Wiley & Sons, Inc. 2015, (ISBN: 978-1-118-61649-9)
Guanbo Wang. Formaldehyde the Swordsman. In: The Republic of Molecules, edited by Y. Ma and F. Yu. Knowledge Publishing House: Beijing, China, 2009, pp. 102-108 (ISBN: 978-7-501-55629-8)
U.S. Patent Granted: Temperature-controlled electrospray ionization source and methods of use thereof, 2014, Patent Number: US8766179
Honors and Awards
2017 Young talents supporting program, China Association for Science and Technology
2017 100-talent Program, Nanjing Normal University
2014 Nomination for CGS/ProQuest Distinguished Dissertation Award, Council of Graduate Schools, USA
2013 Attendance as a Young Researcher at the 63rd Lindau Nobel Laureate Meeting, and the travel grant, Lindau, Germany
2012 Graduate School Fellowship, UMass-Amherst, USA
2011 Marvin D. Rausch Scholarship, Chemistry Dept., UMass-Amherst, USA
2008 Valedictorian, Commencement of College of Chemistry, Peking University, China
2008 Diploma with Honors, Peking University, China
2017 Editorial advisory group member, Cambridge Scholars Publishing, UK
2016 Editorial board member, Analytical Instrumentation (ISSN: 1001-232X)