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Wenjun Tong Professor


Educational Background

PhD, Chemistry, Brown University (2010)

MS, Chemistry, Nanjing University (2003)

BA, Kuang Yaming Honors School, Nanjing University, (2000)



Research Interests

Currently, I am focusing on the separation and detection methods for complex biological components using high performance liquid chromatography, capillary electrophoresis, and tandem mass spectrometer.

I used mutagenesis and modification of DNA and protein to manipulate the positions of DNA-protein crosslinking and determine their interaction motif in the process of Lambda phage recombination. The results showed Integrative and Excisive Recombination had different pathways and formed different intermediates. It solved the long lasting question about how these multi-protein DNA complexes assembled and the related mechanism.

I used scanning tunneling spectroscopy to characterize structure and electronic properties of Self-Assembled Monolayers (SAMs) formed by organic molecules (multi-functionalized anthracene and triptycene molecules, chemically prepared, purified and characterized by variety of techniques including GC/MS, IR, UV-Vis, HPLC, and multi-dimensional NMR) on graphite and of organic molecule devices. Furthermore, organic conformer diodes within SAMs and the capture of nano-particles (NPs) by SAMs were studied. Simulation and analyses of SAMs and graphite were performed using semi-empirical and molecular mechanics (Hyperchem). Additionally, I developed several Windows based applications to facilitate research data processing and analyzing. My work achieved modulation of different morphologies of single component SAM by adjusting the position of ether/gem-difluoride position, realized more complicated pattern with multi-component self-assembly, and illustrated the capability of capturing gold nano-particle by strongly physisorbed SAM.


Research Projects

Research and development of novel continuous HPLC-CE 2D separation technology, Nanjing Zhengchun Tech.


Journal Articles

1. Lingyu Wang, Wenjun Tong*, David D. Y. Chen*, High Resolution Capillary Isoelectric Focusing Mass Spectrometry Analysis of Peptides, Proteins, And Monoclonal Antibodies with a Flow-through Microvial Interface, Analytical Chemistry, 2018, 90(15): 9495-9503

2. Wenjun Tong, Guanbo Wang*, How can native mass spectrometry contribute to characterization of biomacromolecular higher-order structure and interactions? Methods, 2018, 144: 3-13

3. Keke Zhang, Huiying Ma, Ping Yan, Wenjun Tong*, Xiaohua Huang, David D.Y. Chen*, Electrochemical detection of microcystin-LR based on its deleterious effect on DNA, Talanta, 2018, 185: 405-410

4.Tong, W., Warren, D., Seah, N. E., Laxmikanthan, G., Van Duyne, G. D., and Landy, A. (2014). Mapping the λ Integrase Bridges in The Nucleoprotein Holliday Junction Intermediates of Viral Integrative and Excisive Recombination. PNAS. 111(34), 12366-12371.

5. Seah, N. E., Warren, D., Tong, W., Laxmikanthan, G., Van Duyne, G. D., and Landy, A. (2014). Nucleoprotein Architectures Regulating the Directionality of Viral Integration and Excision. PNAS. 111(34), 12372-12377.

Honors and Awards

The Sigma Xi Award for Excellence in Research and High Potential for Further Contributions to Science


Academic Title

Youth Academic Committee, China Association for Instrumental Analysis