PhD, Chemistry, Brown University (2010)
MS, Chemistry, Nanjing University (2003)
BA, Kuang Yaming Honors School, Nanjing University, (2000)
Currently, I am focusing on the separation and detection methods for complex biological components using high performance liquid chromatography, capillary electrophoresis, and tandem mass spectrometer.
I used mutagenesis and modification of DNA and protein to manipulate the positions of DNA-protein crosslinking and determine their interaction motif in the process of Lambda phage recombination. The results showed Integrative and Excisive Recombination had different pathways and formed different intermediates. It solved the long lasting question about how these multi-protein DNA complexes assembled and the related mechanism.
I used scanning tunneling spectroscopy to characterize structure and electronic properties of Self-Assembled Monolayers (SAMs) formed by organic molecules (multi-functionalized anthracene and triptycene molecules, chemically prepared, purified and characterized by variety of techniques including GC/MS, IR, UV-Vis, HPLC, and multi-dimensional NMR) on graphite and of organic molecule devices. Furthermore, organic conformer diodes within SAMs and the capture of nano-particles (NPs) by SAMs were studied. Simulation and analyses of SAMs and graphite were performed using semi-empirical and molecular mechanics (Hyperchem). Additionally, I developed several Windows based applications to facilitate research data processing and analyzing. My work achieved modulation of different morphologies of single component SAM by adjusting the position of ether/gem-difluoride position, realized more complicated pattern with multi-component self-assembly, and illustrated the capability of capturing gold nano-particle by strongly physisorbed SAM.
Research and development of novel continuous HPLC-CE 2D separation technology, Nanjing Zhengchun Tech.
1. Tong, W., Warren, D., Seah, N. E., Laxmikanthan, G., Van Duyne, G. D., and Landy, A. (2014). Mapping the λ Integrase Bridges in The Nucleoprotein Holliday Junction Intermediates of Viral Integrative and Excisive Recombination. PNAS. 111(34), 12366-12371.
2. Seah, N. E., Warren, D., Tong, W., Laxmikanthan, G., Van Duyne, G. D., and Landy, A. (2014). Nucleoprotein Architectures Regulating the Directionality of Viral Integration and Excision. PNAS. 111(34), 12372-12377.
3. Wei, X., Tong, W., Fidler, V., and Zimmt, M. B. (2012). Reactive Capture of Gold Nanoparticles by Strongly Physisorbed Monolayers on Graphite. J. Coll. Interf. Sci., 387(1) 221-227.
4. Tong, W., Xue, Y., and Zimmt, M. B. (2010). Morphology Control and Monolayer Patterning with CF2 Groups: An STM Study. J. Phys. Chem. C 114(48), 20783-20792.
5. Tong, W., Wei, X., and Zimmt, M. B. (2009). Dipolar Control of Monolayer Morphology on Graphite: Self-assembly of Anthracenes with Odd Length Diether Side Chains. J. Phys. Chem C 113(39), 17104-17113.
6. Tong, W., Wei, Y., Armbrust, K. W., and Zimmt, M. B. (2009). Dipolar Side Chain Control of Monolayer Morphology: Symmetrically Substituted 1,5-(Mono- and diether) Anthracenes at the Solution-HOPG Interface. Langmuir 25, 2913-2923.
7. Wei, Y., Tong, W., and Zimmt, M. B. (2008). Self-Assembly of Patterned Monolayers with Nanometer Features: Molecular Selection Based on Dipole Interactions and Chain Length. J. Am. Chem. Soc. 130, 3399-3405.
8. Wei, Y., Tong, W., Wise, C., Wei, X., Armbrust, K., and Zimmt, M. B. (2006). Dipolar Control of Monolayer Morphology: Spontaneous SAM Patterning. J. Am. Chem. Soc. 128, 13362-13363.
Honors and Awards
The Sigma Xi Award for Excellence in Research and High Potential for Further Contributions to Science, Brown University Chapter of Sigma Xi, 2009
Youth Academic Committee, China Association for Instrumental Analysis