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Faculty

Ying Huang Professor

Bio: 

Research Interests

Our group investigates mechanisms involved in the post-transcriptional regulation of mitochondrial gene expressions in fission yeast. We have characterized the mitochondrial tRNA 3-end processing enzyme tRNase Z and Ppr10, which acts as a mitochondrial translational activator. Deletion of either tRNase Z or ppr10 causes apoptotic cell death. Deletion of ppr10 appears to increase the cellular iron concentration and induces oxidative stress. These results indicate a link between mitochondrial dysfunction and iron accumulation, which can cause oxidative stress via the Fenton reaction. Interestingly, it has been suggest that mitochondrial dysfunction, iron accumulation and oxidative stress are linked to Parkinson’s disease (PD). Deletion of ppr10 also cause non-sexual flocculation. We also found that Ppr10 interacts with Mpa1. Our ongoing interests include whether genes involved in flocculation and genes involved in iron homeostasis are co-regulated, how cells sense mitochondrial dysfunction, signal transduction cascades associated with oxidative stress, and how Ppr10 activates mitochondrial translation. We are also interested in the nature of interaction between Ppr10 and Mpa1.

Employment History
Professor, Nanjing Normal University (11/06 - current)
Research Fellow, National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, Maryland, USA (10/98 - present)
Visiting Fellow, NIH, Bethesda, Maryland, USA (10/97 - 9/98)
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai, China (9/86 - 8/92)

Research Projects
1)Characterization of the Schizosaccharomyces pombe tRNA 5’-end processing endonuclease RNase P,National Science Foundation of China (2015.1-2018.12), 
No. 31470778

2)Study on co-regulation of mitochondrial tRNA processing and cell cycle control, National Science Foundation of China (2012.1-20015.12), No. 31170065

3)Characterization of the Schizosaccharomyces pombe tRNA 3’-end processing endonuclease tRNase Z, homolog of putative prostate cancer related protein ELAC2/HsaTRZ2, National Science Foundation of China (2011.1-20013.12), 
No. 3107070

4)Characterization of the Schizosaccharomyces pombe mitochondrial protein Trz2, homolog of putative prostate cancer related protein ELAC2/HsaTRZ2, National Science Foundation of China (2007.1-2009.12), No. 30670446

5)Characterization of the Schizosaccharomyces pombe trz1+ encoding tRNA 3’-end processing endonuclease, homolog of putative prostate cancer related protein   ELAC2/HsaTRZ2, National Science Foundation of China (2008.1-2010.12), 
No. 30771178

Research Papers
1.Wang, Y.,Yan, J., Zhang, Q., Ma, X., Zhang, J., Su, M., Wang, X. and Huang, Y. The Schizosaccharomyces pombe PPR protein Ppr10 associates with a novel protein Mpa1 and acts as a mitochondrial translational activator. Nucleic Acids Res. In press 2017
2.Su, Y., Yang, Y. and Huang, Y. Loss of ppr3, ppr4, ppr6 or ppr10 perturbs iron homeostasis and leads to apoptotic cell death in Schizosaccharomyces pombe. FEBS J.  284, 324-337. (2016)
3.Liu, J., Huang, L., Wang, Y., and Huang, Y. Characterization of cis-elements in the Promoter of trz2 Encoding Schizosaccharomyces pombe Mitochondrial tRNA 3-end Processing Enzyme. Microbiology 163, 75-85 (2016)
4.Su, Y., Chen, C., Huang, L., Yan, J., and Huang, Y. Schizosaccharomyces pombe Homologs of Human DJ-1 Are Stationary Phase-Associated Proteins That Are Involved in Autophagy and Oxidative Stress Resistance. PLOS ONE 10, e0143888.
doi:10.1371/ journal.pone.0143888 (2015)

Contact

E-mail yhuang@njnu.edu.cn
y_shang_hai@126.com
Tel: 025-85891263
Mol: 13951994845 
B.S., East China University of Science and Technology, Shanghai, China
Ph.D., University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA